Inhibition of Ecoli in Sprout Production by Calcinated Calcium

Inhibition of Growth of Escherichiacoli O157:H7 in Fresh Radish (Raphanus sativus L.) Sprout Productionby Calcinated Calcium
Journal of Food Protection: Vol. 62, No. 2, pp. 128-132.

M.L. BARI, H. KUSUNOKI, T. UEMURA, H. FURUKAWA and H. IKEDA

GraduateSchool of Agriculture and Biological Sciences, Osaka Prefecture University

K. ISSHIKI

NationalFood Research Institute, Ibaragi, Japan 

Abstract-Theinhibitory effect of calcinated calcium on the growth of Escherichia coliO157:H7 during fresh radish (Raphanus sativus L.) sprout production wasstudied. It was revealed that the addition of 0.4% (wt/vol) calcinated calciuminto radish sprouting medium which was artificially contaminated with E. coliO157:H7 (3.0 to 3.2 log CFU/ml) completely inhibited the growth or inactivatedthe microorganism. When radish seed extract was used instead of radish sproutproduction, the same extent of growth inhibition or inactivation was observedwith much lower amounts (0.07%) of calcinated calcium under similar experimentalconditions. The findings suggested that calcinated calcium may be useful tocontrol E. coli O157:H7 contamination during the production of radishsprouts.