International Specialty Supply
Supplying Sprout Companies Throughout the World
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International Specialty Supply Supplying Sprout Companies Throughout the World
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820 East 20th Street Cookeville, TN 38501 USA 931 526 1106
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Detection of segments of genetically modified DNA in food processed from soybean 2005 IFT Annual Meeting, July 15-20 - New Orleans, Louisiana Session 36B, Biotechnology: General R. RODRIGUEZ-HERRERA, Food Research Dept. School of Chemistry., Univ. Autónoma de Coahuila, PO Box 252, Saltillo, Coahuila, 25001, Mexico, C. N. Aguilar-Gonzalez, Food Research Dept., Univ. Autonoma de Coahuila, Blvd. V. Carranza e Ing. Jose Cardenas, Saltillo, Coahuila, 25280, Mexico, A. Rodríguez-Vidal, Univ. de Ciencias Quimicas, Food Research Dept. School of Chemistry, PO Box 252, Saltillo, Coahuila, 2500, Mexico, O. Robolloso-Padilla, Univ. Autónoma Agraria Antonio Narro, Dept. of Lacteus, Saltillo, Coahuila, 2500, Mexico, and S. Gonzalez-Morales, Univ. Autonoma de Coahuila, Food Research Dept. School of Chemistry, Prol. Obregón 1685 Col. República pte., Saltillo, Coahuila, 25265, Mexico. At the present time, persistence during soybean-based food processing of genes and genetically modified proteins is unknown. In this study, imported soybean samples from USA were analyzed for presence of genetically modified soybean. Develop an effective methodology for gen and transgenic proteins in soy processed food. In this study, imported soybean samples from USA were analyzed for presence of genetically modified soybean. In this case, soybean seeds were sown and after plant emergence, leaf tissue was collected and DNA was extracted. DNA quality was tested on agarose (1%) gel electrophoresis, after that PCR (Polymerase Chain Reaction) was carried out. The 35s promoter CAMV (Cauliflower mosaic virus) was identified using the 35 S1 and 35S2 pair of primers. The gene EPSPS that confers resistance to herbicide gliphosphate (it codifies for the enzyme 5-enol piruvil shikimate 3-phosphate sintetase) was identified using the RR01 and RR02 pair of primers. The gene Cry 1A confers resistance to insects was identified using the Cry1AB/1AS pair of primers. In addition, genetically modified proteins (Cry 1Ab/ 1Ac and CP4 EPSPS) were identified using Elisa-DAS test. This test is based on a high specificity of antibodies and a high activity of some enzymes (peroxidase) used in this type of trials. Elisa-DAS was performed using the Kit PathoScreen (Agdia) for the genetically modified proteins CP4 EPSPS and Cry 1AB/1AC. Using soybean previously identified as genetically modified, six food products were elaborated (tofu, milk, yogurt, sausage, flour and sprouts). During food processing were obtained samples from processed foods, and from food processing steps were a drastic changes of pH and/or temperature was observed. Results showed presence of promoter and transgenic genes (EPSPS and Cry 1A) in most of the samples. The presence of CP4 EPSPS proteins was detected in all soybean based-foods analyzed except for yogurt and tofu; in contrast Cry 1AB/1AC proteins were was detected in soybean based-foods. About 90% of the imported soybean samples were genetically modified. Transgenic proteins and DNA segments were detected in the tested foods.
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