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Increased growth of Bifidobacterium and Eubacterium by germinated barley
foodstuff, accompanied by enhanced butyrate production in healthy volunteers.
Int J Mol Med. 1999 Feb;3(2):175-9.
Kanauchi O, Fujiyama Y, Mitsuyama K, Araki Y, Ishii T, Nakamura T, Hitomi Y,
Agata K, Saiki T, Andoh A, Toyonaga A, Bamba T.
Applied Bioresearch Center, Corporate Research and Development Division, Kirin
Brewery Co. Ltd., Takasaki, Gunma 370-12, Japan.
Germinated barley foodstuff (GBF) derived from the aleurone and scutellum
fractions of germinated barley mainly consists of low-lignified hemicellulose
and glutamine-rich protein. GBF improves the proliferation of intestinal
epithelial cells and defecation, through the bacterial production of short chain
fatty acids (SCFA), especially butyrate. In this study we investigated the
mechanism of production of butyrate by microflora in humans and in vitro. Daily
administration of 9 g GBF for 14 successive days significantly increased fecal
butyrate content. Fecal Bifidobacterium and Eubacterium were also significantly
increased by GBF administration in healthy volunteers. Ten anaerobic
micro-organisms selected from intestinal microflora were cultured in vitro in
the medium containing GBF as a sole carbon source (GBF medium). After a 3-day
incubation, 7 strains (Bifidobacterium breve, Bifidobacterium longum,
Lactobacillus acidophilus, Lactobacillus casei subsp. casei, Bacteroides ovatus,
Clostridium butyricum, and Eubacterium limosum) lowered the medium pH producing
SCFA. Eubacterium grown together with Bifidobacterium in GBF medium efficiently
produced butyrate. On the other hand, GBF changed the intestinal microflora and
increased probiotics such as Bifidobacterium in the intestinal tract. As a
result, butyrate was produced by the mutual action of Eubacterium and
Bifidobacterium. This butyrate is considered to enhance the proliferation of
colonic epithelial cells.
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