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Purification of sulforaphane from Brassica oleracea seed meal using low-pressure
column chromatography.
J Chromatogr B Analyt
Technol Biomed Life Sci. 2005
Oct 17;
Liang H, Yuan Q,
Xiao Q.
Department of Pharmaceutical Engineering, Beijing University of Chemical
Technology, Beijing 100029, PR China.
Sulforaphane is an isothiocyanate that is present naturally in widely consumed
Brassica oleracea vegetables and has been shown to block the formation of
tumors. The contents of sulforaphane in five groups of B. oleracea seeds
(broccoli, Brussels sprouts, cabbage, cauliflower and kale) were determined by
RP-HPLC using linear gradient of acetonitrile in water. A new low-cost method to
isolate and purify natural sulforaphane from B. oleracea seed meal was described
in this work. Crude sulforaphane was first separated from B. oleracea seed meal
by using immiscible solvent extraction with ethyl acetate, 10% ethanol and
hexane, and the crude sulforaphane was used as raw materials to prepare high
purity sulforaphane by low-pressure column chromatography of silica gel (200-300
mesh) with different eluents and elution modes. Compared with these different
elution methods, the gradient elution was preferable to the isocratic elution
for reducing the elution time and the eluent consumption and increasing the
purity of sulforaphane product. The purity and recovery of sulforaphane were
more than 90% in gradient elution.
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