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Quantitative determination of intact glucosinolates in broccoli, broccoli
sprouts, Brussels sprouts, and cauliflower by high-performance liquid
chromatography-electrospray ionization-tandem mass spectrometry.
Anal Biochem. 2005 Aug 1;343(1):93-9.
Tian Q, Rosselot RA, Schwartz SJ.
Department of Food Science and Technology, The Ohio State University, Columbus,
OH 43210-1007, USA.
Many methods have been proposed to analyze glucosinolates, a class of
phytochemicals whose breakdown products are thought to be responsible for an
improvement in health; however, few are quantitative and many are time
consuming. A selective and sensitive quantitative method for direct
determination of intact glucosinolates was developed using liquid chromatography
coupled to electrospray ionization tandem mass spectrometry with selected
reaction monitoring detection. Detection limits for glucoiberin, sinigrin,
progoitrin, glucoerucin, and glucotropaeolin were 1.75, 1.38, 1.36, 0.6, and
0.63pmol, respectively. Intraassay precision of the method was within 10% for
each compound. The method was successfully applied to quantify 10 individual
glucosinolates in broccoli, broccoli sprouts, Brussels sprouts, and cauliflower.
The advantage of the proposed method includes analysis of individual intact
glucosinolates rather than the conversion to desulfoglucosinolates, an increased
selectivity through the use of mass spectrometry, and a 10-fold improvement in
detection sensitivity over conventionally used HPLC techniques.
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