|
ISS
820 East 20th Street
Cookeville, TN 38501 USA
931 526 1106
Bob@sproutnet.com
中文版
En espaņol
| |
Stimulation of gamma-aminobutyric
acid synthesis activity in brown rice by a chitosan/glutamic acid germination
solution and calcium/calmodulin.
J Biochem Mol Biol. 2003 May 31;36(3):319-25.
Oh SH., Department of Biotechnology, Woosuk University, Jeonju 565-701, Korea.
Changes in the concentrations of gamma-aminobutyric acid (GABA), soluble calcium
ions, glutamic acid, and the activity of glutamate decarboxylase (GAD) were
investigated in non-germinated vs. germinated brown rice. Brown rice was
germinated for 72 h by applying each of the following solutions: (1) distilled
water, (2) 5 mM lactic acid, (3) 50 ppm chitosan in 5 mM lactic acid, (4) 5 mM
glutamic acid, and (5) 50 ppm chitosan in 5 mM glutamic acid. GABA
concentrations were enhanced in all of the germinated brown rice when compared
to the non-germinated brown rice. The GABA concentration was highest in the
chitosan/glutamic acid that germinated brown rice at 2,011 nmol/g fresh
weight, which was 13 times higher than the GABA concentration in the
non-germinated brown rice at 154 nmol/g fresh weight. The concentrations of
glutamic acid were significantly decreased in all of the germinated rice,
regardless of the germination solution. Soluble calcium and GAD were higher in
the germinated brown rice with the chitosan/glutamic acid solution when compared
to the rice that was germinated in the other solutions. GAD that was partially
purified from germinated brown rice was stimulated about 3.6-fold by the
addition of calmodulin in the presence of calcium. These data show that the
germination of brown rice in a chitosan/glutamic acid solution can significantly
increase GABA synthesis activity and the concentration of GABA.
|
