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By knocking out certain Salmonella genes, ARS food safety researchers may discover genes are crucial to this microbe’s ability to attack foods

Amy O. Charkowski

Food Safety and Health Research Unit, Albany, CA

USDA ARS
September 18, 2000

The research, which is being conducted with the sprouted seeds of broccoli, radish, alfalfa, and mung bean, could lead to new and more effective tactics to thwart Salmonella not only in sprouts, but in other fresh produce—and perhaps even in meats and poultry—as well. The ARS investigation into the key Salmonella genes may be unique. So far, the experiments in which a gene or genes have been knocked out of lab strains of the pathogen have yielded a Salmonella that is only one-tenth as effective in colonizing fresh sprouts. Now scientists need to determine which of the 4,000 to 5,000 Salmonella genes are missing or disabled in that strain. The researchers expect that help in answering this question will come from scientists elsewhere who are collaborating in an international effort to determine the makeup and function of all Salmonella genes. Besides adding color, taste, and texture to salads, sandwiches, soups, and other dishes, sprouts provide protein, fiber, and antioxidants such as vitamin C.