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Double Whammy for E. coli and Salmonella
Food & Nutrition Research Briefs, July 1999
USDA ARS

Treating alfalfa seeds and sprouts with a combination of irradiation and chlorine effectively safeguards them against E. coli O157:H7 and Salmonella, researchers found. Not only did the dual treatment kill both organisms, it extended the shelf life of sprouts from about five days to more than a week. This is good news for sprout growers. Since 1995, raw alfalfa sprouts have been recognized as a source of foodborne illness in the United States, with several outbreaks of both E. coli O157:H7 and Salmonella. Since sprouts can't withstand abrasive physical washing because of their fragility, cleaning the seed has become the primary focus.

 

Along with irradiation, the researchers subjected alfalfa seeds to three concentrations of calcium hypochlorite—a chlorine source. A 3-percent weight-per-volume concentration equals about 20,000 parts per million of available chlorine. When the pH is neutral, the 2.5- and 3-percent concentrations reduced E. coli O157:H7 by 99.99 percent. The pH level is important because when the solution is more alkaline, such as pH 10, the chlorine would change to a form that would be less effective against bacteria.

 

According to the scientists, the best way to eliminate pathogens would be a combination of irradiation and sanitation treatments. That's because sprouts and seeds may be contaminated internally, which would prevent a surface disinfectant from working effectively. In the tests, the scientists used the irradiation dose approved for meat. The U.S. Food and Drug Administration has approved ionizing radiation as a safe and effective food preservation tool. The research is part of an effort by a task force of representatives from several federal agencies and industry to find ways to control microbial contamination of sprouts .

 

For more information, contact Donald W. Thayer on irradiation, (215) 233-6582 and William F. Fett on chlorination, (215) 233-6418, Eastern Regional Research Center, Wyndmoor, PA