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International Specialty Supply Supplying Sprout Companies Throughout the World
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An Improved Enrichment Broth for Isolation of Escherichia coli O157, with Specific Reference to Starved Cells, from Radish SproutsApplied and Environmental Microbiology, March 2003, p. 1858-1860, Vol. 69, No. 3Shin Sata,1 Tomohiko Fujisawa,1 Ro Osawa,2* Atsushi Iguchi,2 Shiro Yamai,1 and Toshio Shimada3 Kanagawa Prefectural Public Health Laboratory, Asahi-ku, Yokohama 241-0815,1 Department of Bioscience, Graduate School of Science and Technology, Kobe University, Nada-ku, Kobe 657-8501,2 Department of Bacteriology, National Institute of Infectious Diseases, Toyama, Shinjuku-ku, Tokyo 162-8670, Japan3 Received 8 July 2002/ Accepted 13 December 2002
An enrichment broth was developed for the efficient isolation of Escherichia coli O157 from radish sprouts. The broth was buffered peptone water containing 0.5% sodium thioglycolate (STG-BPW), which was designed to allow growth of E. coli O157 in starved and unstarved states. However, this medium suppressed the growth of non-carbohydrate-fermenting obligate aerobes whose colonial appearance on sorbitol MacConkey agar containing cefixime and tellurite (CT-SMAC) resembled that of E. coli O157. Both starved and unstarved cells of E. coli O157 experimentally inoculated into radish sprouts were successfully recovered with STG-BPW enrichment in all cases, most of which showed marked disappearance of E. coli O157-like colonies on CT-SMAC.
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