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Inhibition of Growth of Escherichia
coli O157:H7 in Fresh Radish (Raphanus sativus L.) Sprout Production
by Calcinated Calcium M.
L. BARI, H. KUSUNOKI, T. UEMURA, H. FURUKAWA and H. IKEDA Graduate
School of Agriculture and Biological Sciences, Osaka Prefecture University K. ISSHIKI National
Food Research Institute, Ibaragi, Japan Abstract—The
inhibitory effect of calcinated calcium on the growth of Escherichia coli
O157:H7 during fresh radish (Raphanus sativus L.) sprout production was
studied. It was revealed that the addition of 0.4% (wt/vol) calcinated calcium
into radish sprouting medium which was artificially contaminated with E. coli
O157:H7 (3.0 to 3.2 log CFU/ml) completely inhibited the growth or inactivated
the microorganism. When radish seed extract was used instead of radish sprout
production, the same extent of growth inhibition or inactivation was observed
with much lower amounts (0.07%) of calcinated calcium under similar experimental
conditions. The findings suggested that calcinated calcium may be useful to
control E. coli O157:H7 contamination during the production of radish
sprouts. |