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Inhibition of Growth of Escherichia coli O157:H7 in Fresh Radish (Raphanus sativus L.) Sprout Production by Calcinated Calcium
Journal of Food Protection: Vol. 62, No. 2, pp. 128–132.

M. L. BARI, H. KUSUNOKI, T. UEMURA, H. FURUKAWA and H. IKEDA

Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University

K. ISSHIKI

National Food Research Institute, Ibaragi, Japan 

Abstract—The inhibitory effect of calcinated calcium on the growth of Escherichia coli O157:H7 during fresh radish (Raphanus sativus L.) sprout production was studied. It was revealed that the addition of 0.4% (wt/vol) calcinated calcium into radish sprouting medium which was artificially contaminated with E. coli O157:H7 (3.0 to 3.2 log CFU/ml) completely inhibited the growth or inactivated the microorganism. When radish seed extract was used instead of radish sprout production, the same extent of growth inhibition or inactivation was observed with much lower amounts (0.07%) of calcinated calcium under similar experimental conditions. The findings suggested that calcinated calcium may be useful to control E. coli O157:H7 contamination during the production of radish sprouts.