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Collaborative study of
the MPN, Anderson-Baird-Parker direct plating, and hydrophobic grid-membrane
filter methods for the enumeration of Escherichia coli biotype I in foods.
Can J Microbiol 1983 Oct;29(10):1247-52
Sharpe AN, Rayman MK, Burgener DM, Conley D, Loit A, Milling M, Peterkin PI,
Purvis U, Malcolm S.
Five Health Protection Branch laboratories compared two membrane filter methods
(the Anderson-Baird-Parker direct plating, and a hydrophobic grid-membrane
filter method) against the most probable number procedure (MPN) for enumerating
Escherichia coli biotype I in foods. Results were available in 24 h by both
membrane filter methods, compared with 10-14 days by the MPN procedure. For
ground beef, Parmesan cheese, and cut green beans, the hydrophobic grid method
generally gave the highest recovery, although the two membrane filter methods
were not significantly different. Both these methods gave significantly higher
recoveries than the MPN procedure, and for most foods, either method would be
preferable. Further work is required before either membrane filter method can be
recommended for bean and alfalfa sprouts, which may contain very high levels of
Klebsiella spp.
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