International Specialty Supply
Supplying Sprout Companies Throughout the World
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International Specialty Supply Supplying Sprout Companies Throughout the World
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820 East 20th Street Cookeville, TN 38501 USA 931 526 1106
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Comparison of Assurance Gold Salmonella
EIA, BAX for
Screening/Salmonella, and GENE-TRAK Salmonella DLP Rapid Assays for Detection of
Salmonella
in Alfalfa Sprouts and Sprout Irrigation Water Diana S. Stewart, Karl F. Reineke, and Mary L. Tortorello U.S. Food and Drug Administration, National Center for Food
Safety and Technology, 6502 S. Archer Rd., Summit-Argo, IL
60501 The Assurance Gold Salmonella EIA, BAX for
Screening/Salmonella, and GENE-TRAK Salmonella DLP rapid assays were compared
with official cultural methods described in the Bacteriological Analytical
Manual (BAM) for analysis of alfalfa sprouts and sprout irrigation water for
the presence of Salmonella. The
lower limits of detection of 4 serovars of Salmonella cells (S.
Tennessee, S. muenchen, S. mbandanka, and S. cubana) in pure culture were
determined as approximately log10 2, 5, and 6 for the BAX, GENE-TRAK,
and Gold EIA, respectively. Despite
its low detection limit, the BAX did not perform as well as the other assays in
analyzing contaminated sprouts and sprout irrigation water.
For 4 different lots of sprouts and sprout irrigation water samples
inoculated with the 4 serovars at low [102 colony forming units (CFU/g)] and
high (68-180 CFU/g) levels, the BAX detected Salmonella in 58/64 (0.6%)
of the samples, compared with 64/64 (100%) by the GENE-TRAK, Gold EIA, and BAM
methods. Assay performance was also
compared for analysis of naturally contaminated sprouts and sprout irrigation
water with 3 lots of alfalfa sprouted seeds associated with different
salmonellosis outbreaks. Positive
assay results for the naturally contaminated samples were Gold EIA 41, GENE-TRAK
36, BAM 33, and BAX 13. |