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Detection and Isolation of Salmonella
from Naturally Contaminated Alfalfa Seeds Following an Outbreak Investigation
J Food Prot 1999 Jun;62(6):662-4
Inami GB, Moler SE.
California Department of Health Services, Microbial Diseases Laboratory,
Berkeley 94704, USA. G_Inami@yahoo.com
Naturally contaminated alfalfa seeds, epidemiologically linked to foodborne
disease outbreaks in Oregon and British Columbia, were tested for the presence
of Salmonella. Ten sample units from the suspected lot were sprouted and grown
for 4 days. After enrichment of the grown sprouts, an enzyme immunoassay (EIA)
and culture method (modified procedure of the Food and Drug Administration
Bacteriological Analytical Manual) were used for the detection and isolation of
Salmonella. Four of the 10 sample units were positive with the EIA; however,
5
of the 10 sample units were culture positive (four were positive for Salmonella
serotype Newport and a fifth was positive for Salmonella serotype Albany and
serotype Schwarzengrund). The positive alfalfa seed sample units were further
tested after shredding, soaking, and washing before culturing. Results suggest
that sprouting and shredding methods may yield greater detection and recovery
rates of Salmonella, but more research with a larger sample size is warranted.
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