|
ISS
820 East 20th Street
Cookeville, TN 38501 USA
931 526 1106
Bob@sproutnet.com
中文版
En espaņol
| |
Effect of
immersion solutions containing enterocin AS-48 on Listeria monocytogenes in
vegetable foods.
Appl Environ Microbiol. 2005 Dec;71(12):7781-7.
Molinos AC, Abriouel H, Ben Omar N, Valdivia E, Lopez RL, Maqueda M, Canamero
MM, Galvez A.
Area de Microbiologia, Departamento de Ciencias de la Salud, Facultad de
Ciencias Experimentales, Universidad de Jaen, Campus Las Lagunillas s/n,
23071-Jaen, Spain. agalvez@ujaen.es.
The effect of immersion solutions containing enterocin AS-48 alone or in
combination with chemical preservatives on survival and proliferation of
Listeria monocytogenes CECT 4032 inoculated on fresh alfalfa sprouts, soybean
sprouts, and green asparagus was tested. Immersion treatments (5 min at room
temperature) with AS-48 solutions (25 microg/ml) reduced listeria counts of
artificially contaminated alfalfa and soybean sprouts by approximately 2.0 to
2.4 log CFU/g compared to a control immersion treatment in distilled water. The
same bacteriocin immersion treatment applied on green asparagus had a very
limited effect. During storage of vegetable samples treated with immersion
solutions of 12.5 and 25 microg of AS-48/ml, viable listeria counts were reduced
below detection limits at days 1 to 7 for alfalfa and soybean sprouts at 6 and
15 degrees C, as well as green asparagus at 15 degrees C. Only a limited
inhibition of listeria proliferation was detected during storage of bacteriocin-treated
alfalfa sprouts and green asparagus at 22 degrees C. Treatment with solutions
containing AS-48 plus lactic acid, sodium lactate, sodium nitrite, sodium
nitrate, trisodium phosphate, trisodium trimetaphosphate, sodium thiosulphate,
n-propyl p-hydroxybenzoate, p-hydoxybenzoic acid methyl ester,
hexadecylpyridinium chloride, peracetic acid, or sodium hypochlorite reduced
viable counts of listeria below detection limits (by approximately 2.6 to 2.7
log CFU/g) upon application of the immersion treatment and/or further storage
for 24 h, depending of the chemical preservative concentration. Significant
increases of antimicrobial activity were also detected for AS-48 plus potassium
permanganate and in some combinations with acetic acid, citric acid, sodium
propionate, and potassium sorbate.
|
