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Microbial quality assessment and pathogen inactivation
by electron beam and gamma irradiation of commercial seed sprouts
25.apr.08
Food Control (Volume 20, Issue 3, March 2009, Pages 200-204)
C.K. Wajea, S.Y. Junb, Y.K. Leeb, B.N. Kimc, D.H. Hanc, C. Jod and J.H. Kwon
Abstract
The microbiological quality of fresh sprouts and their seeds and the potential
use of electron beam and gamma irradiation in inactivating inoculated pathogens
in both samples were evaluated. High levels of aerobic bacteria and coliforms
were enumerated in sprouts. Red radish, alfalfa and broccoli sprouts were
positive for Listeria monocytogenes, while all seed samples were negative for
pathogens. Red radish and broccoli sprouts and their seeds were inoculated with
Escherichia coli O157:H7, Salmonella typhimurium, L. monocytogenes and Bacillus
cereus and irradiated up to 3.0 kGy. The D10 values of the inoculated pathogens
were lower in both broccoli and red radish samples treated with gamma ray than
with electron beam, while the D10 values obtained in seeds were relatively
higher compared with sprouts. This study demonstrated the poor microbiological
quality of commercial sprout and the potential health risk it poses. Irradiation
at appropriate doses is a promising approach for producing safe and
pathogen-free sprouts for consumers.
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