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Isolation of Cyclospora oocysts from fruits and vegetables using lectin-coated
paramagnetic beads
J Food Prot. 2000 Oct;63(10):1410-4
Robertson LJ, Gjerde B, Campbell AT.
Department of Pharmacology, Microbiology and Food Hygiene, The Norwegian School
of Veterinary Science, Oslo.
Published techniques for recovering parasites from fruit and vegetables are
generally inadequate, with low and variable recovery efficiencies. Herein, we
describe an improved method for analyzing fruit and vegetables for Cyclospora
oocysts. The technique includes washing procedures, sonication, and separation
using lectin-coated paramagnetic beads. Identification is by microscopy
(differential interference contrast and fluorescence). Oocyst recovery
efficiencies from mushrooms, lettuce, and raspberries were approximately 12%.
Recovery efficiencies from bean sprouts were approximately 4%. Although no
significant difference in recovery efficiency could be detected between samples
processed using the lectin-coated beads and samples processed without this
procedure, distinct advantages were apparent when the lectin-coated beads were
used. A considerably smaller, cleaner final volume remained for microscopy,
which increases the sensitivity of the technique and reduces operator time.
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