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The occurrence of
salmonellae in bean sprouts in Thailand.
Southeast Asian J Trop Med Public Health 1993 Mar;24(1):114-8
Jerngklinchan J, Saitanu K.
Division of Veterinary Microbiology, Faculty of Veterinary Science,
Chulalongkorn University, Bangkok, Thailand.
Detection of Salmonella spp. in 344 samples of bean sprouts was attempted by
pre-enriched in buffered peptone water (BPW) or lactose broth (LB) at 37 degrees
C, followed by selective enrichments, selenite cystein broth (SCB) and Rappaport
medium (RV) at 43 degrees C. Four selective media, bismuth sulphite agar (BSA),
brilliant green agar (BGA), Salmonella-Shigella agar (SSA) and MacConkey agar
(MA) were used for isolation.
Salmonellae were isolated from 30 (8.7%)
samples. They were
serotypes lexington (56.7%), orion (16.7%), senftenberg (16.7%), tennessee
(3.3%), poona (3.3%) and weltevreden (3.3%). Pre-enrichment in LB, followed by
enrichment in SCB and streaking on to BSA gave the highest recoveries. Fifteen
of 30 strains isolated were sensitive to all 14 antibiotics tested. Seven
strains were resistant to one, and a strains showed multiple resistance to 2-4
antimicrobial agents.
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