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Rapid
combined assay for Salmonella detection in food samples.
Acta Microbiol Immunol Hung 2000;47(4):445-56
Gado I, Major P, Kiraly M, Plaveczky MG.
National Center for Epidemiology B. Johan, Budapest, Hungary.
A rapid method was developed to detect salmonellae in food samples. The method
gave a possibility to obtain results after 28 h 30 min. The preenrichment in
buffered peptone water lasted for 6 h, the enrichment in Rappaport-Vassiliadis
medium was applied for 18 h followed by PCR with INVA1-INVA2 primer pair,
adapting Chiu and Ou's method. This procedure was suitable to demonstrate
salmonella contamination at min. 10 cfu/25 g sample. Out of 18 samples there was
a good agreement between the results of the conventional and rapid methods in
case of 17 samples. PCR with SPVC1-SPVC2 primer pair informing about the
presence of virulence plasmid was performed in separate tubes, because decreased
sensitivity was observed in case of multiplex PCR.
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