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Scale-up seed decontamination process to inactivate Escherichia
coli O157:H7 and Salmonella enteritidis on mung bean seeds
07.sep.09
Foodborne Pathogens and Disease
Md. Latiful Bari, Katsuyoshi Enomoto, Daisuke Nei, Shinnichi Kawamoto
Abstract
A majority of the seed sprout–related outbreaks have been associated with
Escherichia coli O157:H7 and Salmonella spp. Therefore, it is necessary to find
an effective method to inactivate these microorganisms on the seeds before
sprouting. When treatment with hot water at 85°C for 40sec followed by dipping
in cold water for 30sec and soaking into chlorine water (2000ppm) for 2h was
performed, no viable pathogens were found in the enrichment medium and during
the sprouting process. The germination yield of the seed was not affected
significantly (p>0.05). Therefore, these treatments could be useful for the
decontamination method of mung bean seeds intended for sprout production.
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