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Scanning electron microscopy of
native biofilms on mung bean sprouts.
Can J Microbiol. 2003 Jan;49(1):45-50.
Fett WF, Cooke PH.
Food Safety Intervention Technologies Research Unit, Eastern Regional Research
Center, Agricultural Research Service, USDA, Wyndmoor, PA 19038, USA. wfett@arserrc.gov
Native biofilms present on the adaxial surface of cotyledons of mung bean
sprouts (Vigna radiata) were studied by use of scanning electron microscopy.
Biofilms were abundant on the cotyledon surfaces and were comprised of
rod-shaped bacteria, cocci-shaped bacteria, or yeasts, often with one type of
microbe predominant. In contrast to our earlier study of biofilms on green
sprouts (alfalfa, clover, broccoli, and sunflower), yeast and cocci were
abundant on mung bean. Filamentous fungi were not observed. Sheet-like or
fibrillar material (presumably composed of secreted microbial polysaccharides,
proteins, lipids, and nucleic acids) fully or partially covered the biofilms.
Biofilms up to 5 mm in length were observed, and some biofilms were comprised of
more than just a monolayer of microbial cells. Native biofilms on sprout
surfaces undoubtedly play an important role in the ecology of plant epiphytic
microbes and may also afford protected sites for plant and human bacterial
pathogens.
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