Soaked and Germinated Glycine Max
Soaked and germinated Glycine max (soybean seeds) is highly effective blood sugar regulator
Natural Product Radiance, Sep-Oct 2005, ISSN 0972-592X,
Patents (PCT) WO2004/096250, 904/Del/2002 and 663/Del/2003.
Dr. Manju Pathak, B-506, PMO Apartments, C-58/20,Sector-62,
Noida-201301, India.
Tel: +91-120-2402372
Abstract
Glycine max seeds when soaked and germinated, become highly effective blood sugar regulator. This was observed in 35 volunteer type-II diabetes patients. All patients except one were taking oral hypoglycemic drugs (OHG) to control their elevated blood sugar level before they started taking soaked and germinated soybean seeds as a medicine to control their blood sugar. These patients stopped taking OHG during the period of investigation and took only soaked and germinated soybean seeds as a medicine to control their high blood sugar level. It was observed that soaked and germinated soybean seeds are more effective than the OHG. Out of these 35 patients the blood sugar of 22 patients were observed for three months. Out of these 22 patients 4 patients got revived. The high efficacy of such seeds could be because of the following reasons: (1) synthesis of phosphatidylinositol 3 kinase (PI3K) in Glycine max seeds during germination, (2) synthesis of D-chiro inositol during germination and (3) action in synergy of many phytochemicals synthesized/enhanced during germination, which might be making Glycine max seeds, a potent antioxidant.
Introduction
Diabetes is a multidimensional, complex and varied symptom physiological disorder. It is a disease where the sugar level of the blood increases. This research is about an invention of a highly effective blood sugar regulating product from soybean seeds. The aim of this research is to develop a natural product more effective than OHG and free from harmful side effects on the health of diabetes patients.
Subjects, materials and methods:
35 volunteer type-II diabetes patients were investigated in two separate groups to see the effect of soaked and germinated soybean seeds on the blood sugar level. 17 patients were included in group-1 and 18 patients were included in group-2. Their random blood sugar were recorded after six hours of food (lunch) when they were taking OHG. All the patients were taking OHG except one patient before the investigation started. During the investigations these patients of both the groups did not take OHG or any other medicine to control their blood sugar except soaked and germinated soybean seeds. These diabetes patients agreed to stop OHG because their blood sugar level was better controlled by this new medicine. Group-1 patients took two doses (each dose of 250 ml) of soybean milk one in the morning before food and one in the evening before dinner as a medicine (table-1& table-1a). Soybean seeds were soaked for 8 to 12 hours, soaked seeds were ground in fine paste, a milk was prepared from this paste by adding water into it, finally this milk was heated up till boiling. This boiled milk after getting cooled was filtered. This filtered milk was the final form of medicine for group-1 patients. Group-2 patients took two doses (each dose of 15gms) of dry soaked and germinated soybean powder after mixing it in half glass of lukewarm water: one in morning one hour before lunch and one in evening one hour before dinner. Soybean seeds were soaked for 8 to 12 hours, left for 24 hours for germination at room temperature, germinated soybean seeds are dried. These dried seeds were ground in powder and stored as the medicine sample for group-2 patients. In both the groups blood sugar was recorded initially after two hours, followed by other blood sugar measurements after eight hours and twelve hours respectively; then blood sugar was measured once a day; and gradually the frequency of blood sugar measurement was decreased to once a week. Blood sugar was measured for three months in 4 patients of group-1 and in all 18 patients of group-2.
Results:
It was observed that soaking and germination produces high antidiabetes property in simple Glycine max seeds. The production of effective blood sugar regulating property appears to be primarily taking place because of induction of phosphatidylinositol 3 kinase (PI3K) in nodule organogenesis of Glycine max seeds. This natural therapy overcomes the harmful health effects associated with OHG besides being cost effective.
Discussion:
It was observed that their blood sugar was much better controlled than OHG in both the groups of diabetes patients. The results show that this new medicine (soaked and germinated Glycine max seeds) are regulating blood sugar better than OHG. Greater the blood sugar level greater is the fall due to this new medicine. I can think of the following possibilities, which are responsible for the development of the antidiabetes property in soybean seeds during soaking and germination:
1. Synthesis of Phosphatidylinositol 3 Kinase (PI3K) in soybean seed during germination. Phosphatidylinositol 3-kinase (PI3K) is an important component of various receptor tyrosine kinase complexes in mammalian cells. PI3K plays a central role in insulin’s metabolic effect. PI3Kcatalyzes the generation of phosphatidyl inositol (3,4,5)triphosphate (PIP(3)). Inhibition of PI3K activity results in blockade of insulin signaling including glucose uptake and glycogen synthesis. This, PIP(3) is a critical mediator of insulin action. Either this soybean PI3K is catalyzing the reactions to facilitate the proper reactions to achieve insulin’s physiologic expression or it is helping in synthesis of appropriate phosphatidyl inositol derivative, which might be mediating insulin’s physiologic expression. I am evaluating both the possibilities. ( ref. 1- 5 )
2. Synthesis of D-chiro inositol in soybean seeds during embryo development could be producing antidiabetes property in soaked and germinated soybean seeds. ( ref. -6,7 )
3. Enhancement of vitamins during soaking and germination of soybean seed, particularly vitamins of B group (niacin and riboflavin) could be playing important roles. (a) The vitamins enriched soaked and germinated soybean seeds could be acting as good superoxide scavengers. Recent studies report that overproduction of superoxide by the mitochondrial electron transport chain seems to be the first and key event in the activation of all other pathways involved in the pathogenesis of diabetic complications (ref-8). (b) Intracellular concentration of NAD (nicotinamide adenine dinucleotide ) is depleted in diabetes (ref-8 ) which slows the rate of glycolysis. Niacin, increased during embryo development of soybean seeds, could be increasing the intracellular level of NAD and hence increasing the rate of glycolysis. Vitamins are organic nutrients that are required in small quantities for a variety of biochemical functions, and which generally, cannot be synthesized by the body and must therefore be supplied by the diet.
Apart from the above possible factors on the basis of published research papers, I also think on my own that during diabetes human body might be going to lower energy state and when a diabetes patient takes soaked and germinated soybean powder the energy state of the body goes up. I think this low energy state of the human body is the vital factor for many physiological complications including reduced activation of many enzymes in the body.
Looking at the results it can be concluded that soaking and early germination converts simple Glycine max seeds into an effective blood sugar regulator. This natural therapy overcomes the harmful health effects associated with OHG besides being cost effective.
References
1. Hong Z, Verma DP (1994) A phosphatidylinositol 3-kinase is induced during soybean nodule organogenesis and is associated with membrane proliferation. Proc Natl Acad Sci USA 91(20): 9617-21
2. Lochhead PA, Coghlan M, Rice SQ, Sutherland C (2001) Inhibition of GSK-3 selectively reduces glucose-6-phosphatase and phosphatase and phosphoenolypyruvate carboxykinase gene expression. Diabetes 50(5): 937-46
3. Baumgartener JW (2003) SHIP2: an emerging target for the treatment of type 2 diabetes mellitus. Curr Drug Targets Immune Endocr Metabol Disord 3(4): 291-8
4. Bouzakri K, Roques M, Gual P, Espinosa S, Guebre-Egziabher F, Riou JP, Laville M, Le Marchand-Brustel Y, Tanti JF, Vidal H (2003) Reduced activation of phosphatidylinositol-3 kinase and increased serine 636 phosphorylation of insulin receptor substrate-1 in primary culture of skeletal muscle cells from patients with type 2 diabetes. Diabetes 52(6): 1319-25
5. Hori H, Sasaoka T, Ishihara H, Wada T, Murakami S, Ishiki M, Kobayashi M (2002) Association of SH2-containing inositol phosphatase 2 with the insulin resistance of diabetic db/db mice. Diabetes 51(8): 2387-94
6. Larner J (2002) D-chiro-inositol–its functional role in insulin action and Its deficit in insulin resistance. Int J Exp Diabetes Res 3(1): 47-60
7. Guoqiao Jiang, Ammulu Hari Krishnan, Yong-Woong Kim, Thomas J. Wacek, and Hari B. Krishnan (2001) Functional myo-Inositol Dehydrogenase Gene Is Required for Efficient Nitrogen Fixation. Journal of Bacteriology 183 (8): 2595-2604
8. Ceriello A (2003) New insights on oxidative stress and diabetic complications may lead to a “causal” antioxidant therapy. Diabetes Care 26(5): 1589-96
Table – 1
Effect of 250ml Soaked Soybean solution (SSS) on the blood sugar after two hours of its intake
(On group-1 patients)
Patient | Age at detection of diabetes | Present Age | Random Blood sugar (after six hours of the lunch) in mg/dl when they were on OHG | Blood Sugar in mg/dl after two hours of intake of SSS | Reduction in blood sugar in mg/dl due to SSS |
1 | 45 | 50 | 134 | 123 | 11 |
2 | 63 | 70 | 294 | 109 | 185 |
3 | 36 | 58 | 141 | 110 | 31 |
4 | 68 | 70 | 477 | 409 | 68 |
5 | 46 | 46 | 355 | 290 | 65 |
6 | 45 | 52 | 249 | 172 | 77 |
7 | 43 | 48 | 170 | 140 | 30 |
8 | 37 | 40 | 196 | 106 | 90 |
9 | 38 | 44 | 121 | 104 | 17 |
10 | 36 | 45 | 166 | 123 | 43 |
11 | 40 | 45 | 96 | 75 | 21 |
12 | 43 | 43 | 152 | 115 | 37 |
13 | 40 | 49 | 232 | 214 | 18 |
14 | 38 | 42 | 135 | 84 | 51 |
15 | 40 | 43 | 89 | 63 | 26 |
16 | 43 | 45 | 86 | 116 | -30 |
17 | 30 | 37 | 76 | 84 | -8 |
OHG = Oral Hypoglycemic Drug Random Blood Sugar = Blood sugar taken after six hours of food. |
Table -1 (a)
The effect of Soaked Soybean Solution (SSS) on blood sugar for four months on patients taking SSS twice a day (250ml per dose) after stopping OHG.
(On group-1 patients)
Patients of Table 1 | Previous random blood Sugar in mg/dl when the patient was taking OHG medicine for controlling blood sugar | Blood Sugar in mg/dl after the start of SSS as a medicine and after stopping other OHG for controlling blood sugar | |||
Aug 2002 Fasting | Sept 2002 Fasting | Oct 2002 Fasting | Nov 2002 Fasting | ||
5 | Detected Diabetic for the first time in Aug 2002. 355 | After 10 Days 121 | 103 | 109 | 106 |
13 | 232 | 134 | 100 | 117 | 126 |
11 | 96 | 89 | 108 | 118 | 106 |
12 | 152 | 118 | 115 | 92 | 108 |
OHG = Oral Hypoglycemic Drug Random Blood Sugar = Blood sugar taken after six hours of food |
Table – 2
Effect of Soaked Soybean Powder (SSP) on blood sugar after two hours of its intake after mixing it in a half glass of lukewarm water
(On group-2 patients)
Patient | Sex | Age at detection of diabetes | Present Age | Random Blood sugar** (after six hours of lunch) in mg/dl when they were on OHG* | Dosage of SSP in gms | Blood Sugar in mg/dl after two hours of intake of SSP | Reduction in blood sugar in mg/dl due to SSP |
1 | M | 40 | 46 | 264 | 15 | 165 | 99 |
2 | M | 44 | 47 | 315 | 15 | 197 | 118 |
3 | F | 44 | 45 | 145 | 7.5 | 128 | 17 |
4 | M | 45 | 52 | 230 | 15 | 155 | 75 |
5 | M | 42 | 42 | 168 | 15 | 98 | 70 |
6 | M | 57 | 59 | 235 | 15 | 82 | 153 |
7 | M | 45 | 47 | 149 | 15 | 109 | 40 |
8 | M | 42 | 47 | 294 | 7.5 | 159 | 135 |
9 | M | 37 | 51 | 173 | 15 | 106 | 67 |
10 | M | 52 | 58 | 237 | 15 | 156 | 81 |
11 | F | 37 | 51 | 321 | 15 | 149 | 172 |
12 | F | 56 | 58 | 212 | 15 | 147 | 65 |
13 | M | 44 | 46 | 316 | 15 | 305 | 11 |
14 | M | 51 | 59 | 253 | 15 | 173 | 80 |
15 | F | 46 | 51 | 384 | 15 | 294 | 90 |
16 | M | 40 | 45 | 191 | 15 | 120 | 71 |
17 | F | 43 | 47 | 145 | 7.5 | 128 | 17 |
18 | F | 48 | 50 | 262 | 15 | 167 | 95 |
OHG = Oral Hypoglycemic Drug Random Blood Sugar = Blood sugar taken after six hours of food |
Table – 2 (a )
The effect of Soaked Soybean Powder (SSP) on blood sugar for three months on patients taking SSP twice a day (15 gms per dose) after stopping OHG.
(On group-2 patients)
Patients of Table-2 | Age | Previous blood sugar in mg/dl when patients were taking OHG drugs | Blood sugar in mg/dl after patients started SSP only as a blood sugar controlling medicine | ||||||||||
After 15 Days | After 1 Month | After 2 months | After 3 months | ||||||||||
ADD | ATM | PA | F | PP | F | PP | F | PP | F | PP | F | PP | |
1 | 40 | 40 | 46 | 140 | 264 | 130 | 243 | 112 | 153 | 95 | 210 | 96 | 140 |
2 | 44 | 44 | 47 | 148 | 361 | 135 | 254 | 140 | 232 | 130 | 220 | 122 | 212 |
3 | 44 | 44 | 45 | 150 | 210 | 118 | 145 | 108 | 122 | 85 | 130 | 80 | 120 |
4 | 45 | 45 | 52 | 190 | 235 | 150 | 191 | 134 | 150 | 121 | 146 | 116 | 144 |
5 | 42 | 42 | 42 | 120 | 168 | 110 | 168 | 97 | 151 | 88 | 134 | 73 | 106 |
6 | 57 | 57 | 59 | 188 | 210 | 96 | 150 | 92 | 143 | 84 | 132 | 85 | 124 |
7 | 45 | 45 | 47 | 119 | 149 | 110 | 132 | 87 | 122 | 93 | 144 | 102 | 108 |
8 | 42 | 42 | 47 | 198 | 294 | 179 | 271 | 164 | 258 | 139 | 237 | 152 | 240 |
9 | 37 | 37 | 51 | 106 | 173 | 96 | 173 | 87 | 118 | 106 | 127 | 84 | 120 |
10 | 52 | 52 | 58 | 155 | 237 | 156 | 237 | 157 | 189 | 158 | 190 | 131 | 189 |
11 | 37 | 37 | 51 | 152 | 321 | 148 | 165 | 140 | 188 | 139 | 155 | 126 | 150 |
12 | 56 | 56 | 58 | 174 | 325 | 194 | 310 | 168 | 201 | 175 | 295 | 164 | 210 |
13 | 44 | 44 | 46 | 277 | 316 | 262 | 298 | 251 | 284 | 236 | 273 | 186 | 239 |
14 | 51 | 51 | 59 | 168 | 293 | 174 | 281 | 158 | 264 | 163 | 273 | 157 | 261 |
15 | 46 | 46 | 51 | 221 | 384 | 190 | 35 | 185 | 321 | 156 | 271 | – | – |
16 | 40 | 40 | 45 | 150 | 191 | 161 | 210 | 134 | 150 | 121 | 146 | 124 | 160 |
17 | 43 | 44 | 47 | 130 | 173 | 107 | 112 | 108 | 122 | 85 | 130 | 80 | 120 |
18 | 48 | 48 | 50 | 156 | 262 | 145 | 232 | 133 | 195 | 121 | 155 | 107 | 164 |
ADD = Age at Diabetes Detected ATM = Age at Medicine ( OHG ) Taken for control of the blood sugar PA = Present Age |