Soaked and Germinated Glycine Max

Soaked and germinated Glycine max (soybean seeds) is highly effective blood sugar regulator

Natural Product Radiance, Sep-Oct 2005, ISSN 0972-592X, 

Patents (PCT) WO2004/096250, 904/Del/2002 and 663/Del/2003.

Dr. Manju Pathak,  B-506, PMO Apartments, C-58/20,Sector-62,

Noida-201301, India.

Tel: +91-120-2402372

Abstract

Glycine max seeds when soaked and germinated, become highly effective blood sugar regulator. This was observed in 35 volunteer type-II diabetes patients. All patients except one were taking oral hypoglycemic drugs (OHG) to control their elevated blood sugar level before they started taking soaked and germinated soybean seeds as a medicine to control their blood sugar. These patients stopped taking OHG during the period of investigation and took only soaked and germinated soybean seeds as a medicine to control their high blood sugar level. It was observed that soaked and germinated soybean seeds are more effective than the OHG. Out of these 35 patients the blood sugar of 22 patients were observed for three months. Out of these 22 patients 4 patients got revived. The high efficacy of such seeds could be because of the following reasons: (1) synthesis of phosphatidylinositol 3 kinase (PI3K) in Glycine max seeds during germination, (2) synthesis of D-chiro inositol during germination and (3) action in synergy of many phytochemicals synthesized/enhanced during germination, which might be making Glycine max seeds, a potent antioxidant.  

Introduction

Diabetes is a multidimensional, complex and varied symptom physiological disorder. It is a disease where the sugar level of the blood increases. This research is about an invention of a highly effective blood sugar regulating product from soybean seeds. The aim of this research is to develop a natural product more effective than OHG and free from harmful side effects on the health of diabetes patients. 

Subjects, materials and methods:

35 volunteer type-II diabetes patients were investigated in two separate groups to see the effect of soaked and germinated soybean seeds on the blood sugar level. 17 patients were included in group-1 and 18 patients were included in group-2. Their random blood sugar were recorded after six hours of food (lunch) when they were taking OHG. All the patients were taking OHG except one patient before the investigation started. During the investigations these patients of both the groups did not take OHG or any other medicine to control their blood sugar except soaked and germinated soybean seeds. These diabetes patients agreed to stop OHG because their blood sugar level was better controlled by this new medicine. Group-1 patients took two doses (each dose of 250 ml) of soybean milk one in the morning before food and one in the evening before dinner as a medicine (table-1& table-1a). Soybean seeds were soaked for 8 to 12 hours, soaked seeds were ground in fine paste, a milk was prepared from this paste by adding water into it, finally this milk was heated up till boiling. This boiled milk after getting cooled was filtered. This filtered milk was the final form of medicine for group-1 patients. Group-2 patients took two doses (each dose of 15gms) of dry soaked and germinated soybean powder after mixing it in half glass of lukewarm water: one in morning one hour before lunch and one in evening one hour before dinner. Soybean seeds were soaked for 8 to 12 hours, left for 24 hours for germination at room temperature, germinated soybean seeds are dried. These dried seeds were ground in powder and stored as the medicine sample for group-2 patients. In both the groups blood sugar was recorded initially after two hours, followed by other blood sugar measurements after eight hours and twelve hours respectively; then blood sugar was measured once a day; and gradually the frequency of blood sugar measurement was decreased to once a week. Blood sugar was measured for three months in 4 patients of group-1 and in all 18 patients of group-2. 

Results:

It was observed that soaking and germination produces high antidiabetes property in simple Glycine max seeds. The production of effective blood sugar regulating property appears to be primarily taking place because of induction of phosphatidylinositol 3 kinase (PI3K) in nodule organogenesis of Glycine max seeds. This natural therapy overcomes the harmful health effects associated with OHG besides being cost effective. 

Discussion:

It was observed that their blood sugar was much better controlled than OHG in both the groups of diabetes patients. The results show that this new medicine (soaked and germinated Glycine max seeds) are regulating blood sugar better than OHG. Greater the blood sugar level greater is the fall due to this new medicine. I can think of the following possibilities, which are responsible for the development of the antidiabetes property in soybean seeds during soaking and germination:

1.     Synthesis of Phosphatidylinositol 3 Kinase (PI3K) in soybean seed during germination. Phosphatidylinositol 3-kinase (PI3K) is an important component of various receptor tyrosine kinase complexes in mammalian cells. PI3K plays a central role in insulin's metabolic effect. PI3Kcatalyzes the generation of phosphatidyl inositol (3,4,5)triphosphate (PIP(3)). Inhibition of PI3K activity results in blockade of insulin signaling including glucose uptake and glycogen synthesis. This, PIP(3) is a critical mediator of insulin action. Either this soybean PI3K is catalyzing the reactions to facilitate the proper reactions to achieve insulin's physiologic expression or it is helping in synthesis of appropriate phosphatidyl inositol derivative, which might be mediating insulin's physiologic expression. I am evaluating both the possibilities. ( ref. 1- 5 ) 

2.     Synthesis of D-chiro inositol in soybean seeds during embryo development could be producing antidiabetes property in soaked and germinated soybean seeds. ( ref. -6,7 ) 

3.     Enhancement of vitamins during soaking and germination of soybean seed, particularly vitamins of B group (niacin and riboflavin) could be playing important roles. (a) The vitamins enriched soaked and germinated soybean seeds could be acting as good superoxide scavengers. Recent studies report that overproduction of superoxide by the mitochondrial electron transport chain seems to be the first and key event in the activation of all other pathways involved in the pathogenesis of diabetic complications (ref-8). (b) Intracellular concentration of NAD (nicotinamide adenine dinucleotide ) is depleted in diabetes (ref-8 ) which slows the rate of glycolysis. Niacin, increased during embryo development of soybean seeds, could be increasing the intracellular level of NAD and hence increasing the rate of glycolysis. Vitamins are organic nutrients that are required in small quantities for a variety of biochemical functions, and which generally, cannot be synthesized by the body and must therefore be supplied by the diet. 

Apart from the above possible factors on the basis of published research papers, I also think on my own that during diabetes human body might be going to lower energy state and when a diabetes patient takes soaked and germinated soybean powder the energy state of the body goes up. I think this low energy state of the human body is the vital factor for many physiological complications including reduced activation of many enzymes in the body.  

Looking at the results it can be concluded that soaking and early germination converts simple Glycine max seeds into an effective blood sugar regulator. This natural therapy overcomes the harmful health effects associated with OHG besides being cost effective.  

References 

1.     Hong Z, Verma DP (1994) A phosphatidylinositol 3-kinase is induced during soybean nodule organogenesis and is associated with membrane proliferation.  Proc Natl Acad Sci USA 91(20): 9617-21

 

2.   Lochhead PA, Coghlan M, Rice SQ, Sutherland C (2001) Inhibition of GSK-3 selectively reduces glucose-6-phosphatase and phosphatase and phosphoenolypyruvate carboxykinase gene expression. Diabetes  50(5): 937-46

 

3.   Baumgartener JW (2003) SHIP2: an emerging target for the treatment of type 2 diabetes mellitus. Curr Drug Targets Immune Endocr Metabol Disord 3(4): 291-8

 

4.   Bouzakri K, Roques M, Gual P, Espinosa S, Guebre-Egziabher F, Riou JP, Laville M, Le Marchand-Brustel Y, Tanti JF, Vidal H (2003) Reduced activation of phosphatidylinositol-3 kinase and increased serine 636 phosphorylation of insulin  receptor substrate-1 in primary culture of skeletal muscle cells from patients with type 2 diabetes. Diabetes 52(6): 1319-25

 

5.    Hori H, Sasaoka T, Ishihara H, Wada T, Murakami S, Ishiki M, Kobayashi M (2002) Association of SH2-containing inositol phosphatase 2 with the insulin resistance of diabetic db/db mice. Diabetes 51(8): 2387-94

 

6.    Larner J (2002) D-chiro-inositol--its functional role in insulin action and Its deficit in insulin resistance. Int J Exp Diabetes Res 3(1): 47-60

 

7.    Guoqiao Jiang, Ammulu Hari Krishnan, Yong-Woong Kim, Thomas J. Wacek, and Hari B. Krishnan (2001) Functional myo-Inositol Dehydrogenase Gene Is Required for Efficient Nitrogen Fixation. Journal of Bacteriology 183 (8): 2595-2604

 

8.    Ceriello A (2003) New insights on oxidative stress and diabetic complications may lead to a "causal" antioxidant therapy. Diabetes Care 26(5): 1589-96

 

  

Table - 1

Effect of 250ml Soaked Soybean solution (SSS) on the blood sugar after two hours of its intake

(On group-1 patients)

Patient

Age at detection

of

diabetes

Present Age

Random Blood sugar (after six hours of the lunch) in mg/dl when they were on OHG

Blood Sugar in mg/dl after two hours of intake of  SSS

Reduction in

blood sugar in mg/dl due to SSS

1

45

50

134

123

11

2

63

70

294

109

185

3

36

58

141

110

31

4

68

70

477

409

68

5

46

46

355

290

65

6

45

52

249

172

77

7

43

48

170

140

30

8

37

40

196

106

90

9

38

44

121

104

17

10

36

45

166

123

43

11

40

45

96

75

21

12

43

43

152

115

37

13

40

49

232

214

18

14

38

42

135

84

51

15

40

43

89

63

26

16

43

45

86

116

-30

17

30

37

76

84

-8

OHG = Oral Hypoglycemic Drug

Random Blood Sugar = Blood sugar taken after six hours of food. 

  

Table -1 (a)

The effect of Soaked Soybean Solution (SSS) on blood sugar for four months on patients taking SSS  twice a day (250ml per dose) after stopping OHG.

(On group-1 patients)

 

Patients of Table 1

Previous random blood Sugar in mg/dl when the patient was taking OHG medicine for controlling blood sugar

Blood Sugar in mg/dl after the start of SSS as a medicine and after stopping other OHG for controlling blood sugar

Aug 2002

Fasting

Sept 2002

Fasting

Oct 2002

Fasting

Nov 2002

Fasting

 

 

5

Detected Diabetic for the first time in Aug 2002.

355

After 10

Days

121

 

 

103

 

 

109

 

 

106

13

232

134

100

117

126

11

96

89

108

118

106

12

152

118

115

92

108

OHG = Oral Hypoglycemic Drug

Random Blood Sugar = Blood sugar taken after six hours of food

 

Table - 2

Effect of Soaked Soybean Powder (SSP) on blood sugar after two hours of its intake after mixing it in a half glass of lukewarm water

(On group-2 patients)

 

Patient

Sex

Age at detection of diabetes

Present Age

Random Blood sugar** (after six hours of lunch) in mg/dl when they were on OHG*

Dosage of SSP

in gms

Blood Sugar in mg/dl after two hours of intake of SSP

Reduction in blood sugar in mg/dl due to SSP

1

M

40

46

264

15

165

99

2

M

44

47

315

15

197

118

3

F

44

45

145

7.5

128

17

4

M

45

52

230

15

155

75

5

M

42

42

168

15

98

70

6

M

57

59

235

15

82

153

7

M

45

47

149

15

109

40

8

M

42

47

294

7.5

159

135

9

M

37

51

173

15

106

67

10

M

52

58

237

15

156

81

11

F

37

51

321

15

149

172

12

F

56

58

212

15

147

65

13

M

44

46

316

15

305

11

14

M

51

59

253

15

173

80

15

F

46

51

384

15

294

90

16

M

40

45

191

15

120

71

17

F

43

47

145

7.5

128

17

18

F

48

50

262

15

167

95

OHG = Oral Hypoglycemic Drug

Random Blood Sugar = Blood sugar taken after six hours of food

  

Table - 2 (a )

The effect of Soaked Soybean Powder (SSP) on blood sugar for three months on patients taking SSP twice a day (15 gms per dose) after stopping OHG.

(On group-2 patients) 

 Patients of Table-2

 

Age

 Previous blood sugar in mg/dl when patients were taking OHG drugs

Blood sugar in mg/dl after patients started SSP only as a blood sugar controlling medicine

After 15 Days

After 1 Month

After 2 months

After 3 months

ADD

ATM

PA

F

PP

F

PP

F

PP

F

PP

F

PP

1

40

40

46

140

264

130

243

112

153

95

210

96

140

2

44

44

47

148

361

135

254

140

232

130

220

122

212

3

44

44

45

150

210

118

145

108

122

85

130

80

120

4

45

45

52

190

235

150

191

134

150

121

146

116

144

5

42

42

42

120

168

110

168

97

151

88

134

73

106

6

57

57

59

188

210

96

150

92

143

84

132

85

124

7

45

45

47

119

149

110

132

87

122

93

144

102

108

8

42

42

47

198

294

179

271

164

258

139

237

152

240

9

37

37

51

106

173

96

173

87

118

106

127

84

120

10

52

52

58

155

237

156

237

157

189

158

190

131

189

11

37

37

51

152

321

148

165

140

188

139

155

126

150

12

56

56

58

174

325

194

310

168

201

175

295

164

210

13

44

44

46

277

316

262

298

251

284

236

273

186

239

14

51

51

59

168

293

174

281

158

264

163

273

157

261

15

46

46

51

221

384

190

35

185

321

156

271

-

-

16

40

40

45

150

191

161

210

134

150

121

146

124

160

17

43

44

47

130

173

107

112

108

122

85

130

80

120

18

48

48

50

156

262

145

232

133

195

121

155

107

164

ADD = Age at Diabetes Detected ATM = Age at Medicine ( OHG ) Taken for control of the blood sugar

PA = Present Age