Seed Sanitation - Chlorine Sprout Safety Research

We have amassed helpful articles on seed sanitation, chlorine and sprout safety. Enjoy this research and get in touch if you have any questions.

Also see our Links Page

"Alfalfa Seed Decontamination in a Salmonella Outbreak", CDC, Emerging Infectious Diseases, April 2003, Vol 9, No.4, Christopher J. Gill, William E. Keene, Janet C. Mohle-Boetani, Jeff A. Farrar, Patti L. Waller, Christine G. Hahn, and Paul R. Cieslak.

"Application of calcium hypochlorite as a seed disinfectant (a preliminary report)", K Jinnoh,  et al., 1975, Hyogo Kenritsu Nogyo Shikenjo Kenkyu Hokoku, 23, 1-8.

"Calcium hypochlorite as a disinfectant for seeds", Jinno, K , et al., 1977, Nogyo Oyobi Engei, 52, ,1489-1494.

"Comment on Produce Safety from Production to Consumption", Posted July 22, 2004, Food and Drug Administration Docket No. 2004M-0258. International Specialty Supply.

"Chlorine Inactivation of Eschrichia Coli O157:H7 in Water", October 2001, Journal of Food Protection: Vol. 64, No. 10, pp. 16071609., Tong Zhao, Michael P. Doyle, and Ping Zhao, Center for Food Safety, University of Georgia, Paul Blake, Georgia State Public Health Laboratory, Georgia State Public Health Department, Fone-Mao Wu, Center for Food Safety, University of Georgia.

"Curing of Salmonella Enterica, Serovar Typhimurium - Contaminated Cowpea Seeds and Sprouts with Vinegar and Chlorination" Journal of Food Processing & Preservation; Volume 29 Issue 3-4 Page 268  - June 2005; doi:10.1111/j.1745-4549.2005.00027.x; Bhoj Raj Singh, Mudit Chandra, Ravikant Agarwal, and Nagarajan Babu.

"Disinfection of rice seeds prior to sprouting" J. Food Sci. 62:611-615. 1997. Piernas, V. and J.P. Guiraud.

"Disinfection of seed surfaces with sodium hypochlorite", Sauer et al, Phytopathology, 1986, vol. 76 no. 7, pp. 745-749.

"Effect of sodium hypochlorite treatment on germination of spinach seeds", Ku, J H., et al., Han'Guk Wonye Hakhoechi, 37, (1996),357-361.

"Effect of sodium hypochlorite treatment on seed-borne organisms and germination of imported vegetable seeds", S. Lo, (1973), Chih Wu Pao Hu Hsueh Hui Hui K'an, 15, 147-152.

"Effectiveness of Acid/Chlorine rinses at Eliminating E. coli O157:H7 from Alfalfa Seeds Prior to Sprouting", Megan Lang, Steve Ingham, Barbara Ingham; University of Wisconsin-Madison

"Factors affecting the efficacy of chlorine against Esherichia coli O157:H7 and Salmonella on alfalfa seed" Food Microbiology, Vol. 19, No. 2/3, April/June 2002, ISSN: 0740-0020, EISSN: 1095-9998 , W. F. Fett  , pp. 135-149.

"Inactivation of Escherichia coli O157:H7 on Radish Seeds by Sequential Treatments with Chlorine Dioxide, Drying, and Dry Heat without Loss of Seed Viability."Appl Environ Microbiol. 2011 Sep;77(18):6680-6. Epub 2011 Jul 29.

Bang J,Kim H, Kim H, Beuchat LR, Ryu JH. Graduate School of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-ku, Seoul 136-701, Republic of Korea.

"Microbial Quality of Chlorine Soaked Mung Bean Seeds and Sprouts" Food Sci. Technol. Res., 11 (1), 95-100, 2005, Alonzo A. GABRIEL, Department of Food Science and Nutrition, College of Home Economics, University of the Philippines.

"Reduction of Escherichia Coli O157:H7 and Salmonella spp. on Laboratory-Inoculated Mung Bean Seed by Chlorine Treatment", May 2002, Journal of Food Protection: Vol. 65, No. 5, pp. 848­852., William F. Fett, Eastern Regional Research Center, Agricultural Research Service, U.S.D.A

"Seed disinfection with hypochlorites: A selected literature review of hypochlorite chemistry and definition of terms", Abdul-Baki, A , et al., 1979, J. Seed Technol., 4, 43-56.

"Sodium hypochlorite: effect of solution pH on rice seed disinfestation and its direct effect on seeding growth", Chun, S C., et al., Plant Dis., 81, 1997, 821-824.

"Synergistic effect of chlorine dioxide and drying treatments for inactivating Escherichia coli O157:H7 on radish seeds." J Food Prot. 2010 Jul;73(7):1225-30. Kim H, Kim H, Bang J, Beuchat LR, Ryu JH. Division of Human Environmental Sciences, Wonkwang University, Shinyong-dong, Iksan, Jeonbuk, Republic of Korea.